安徽农业科学2009，Vol.37Issue(30)：14611-14613,14634,4.
枸杞基因组DNA的提取及RAPD反应体系的优化
Extraction of Chinese Wolfberry Genomic DNA and Optimization of its RAPD Reaction System
张磊 1任贤1
作者信息
- 1. 北方民族大学生物科学与工程学院,宁夏,银川,750021
- 折叠
摘要
Abstract
[Objective] The extraction of Chinese wolfberry genomic DNA and optimization of its RAPD reaction system were studied. [Method] The concentration range of Chinese wolfberry genomic DNA extracted with optimal CTABMethodwas tested and the RAPD reaction system was further optimized scientifically and reasonably. [Results] The concentration range of Chinese wolfberry genomic DNA was among 3.5-5. 5 μg/μl, which could fully met the demand of RAPD-PCR. [Conclusion] This way has the advantage of few steps, low-cost and high-efficiency, which is more suitable for the Chinese wolfberry genomic DNA extraction.关键词
枸杞/基因组DNA:RAPD/优化Key words
Chinese wolfberry/Genomic DNA/RAPD/Optimization分类
农业科技引用本文复制引用
张磊,任贤..枸杞基因组DNA的提取及RAPD反应体系的优化[J].安徽农业科学,2009,37(30):14611-14613,14634,4.
