安徽农业科学2009，Vol.37Issue(30)：14629-14631,3.
冬虫夏草无性型原生质体再生条件研究
Study on Protoplast Regeneration Conditions of Anamorphic Strains of Cordyceps sinensis
摘要
Abstract
[Objective] The research aimed to select the optimum extracting procedure for regenerations of protoplast of anamorphic strains of Cordyceps sinensis. [Method] The optimum extracting procedure was studied through one-factor test. The effects of cellulose enzyme concentration, snailase concentration, volumetric ratio of them, hydrolytic temperature, hydrolytic time, mycelia age, stabilizer and culture medium on the regeneration rate of protoplast of anamorphic strains were studied. [Result] The optimum extracting procedure of the regeneration conditions of protoplast were as follow; Concentration of cellulose enzyme was 0.5% , concentraction of snailase was 0.5 % , volumetric ratio of them was 1: 1, hydrolytic temperature was 35 ℃ , hydrolytic time was 1.5 h, mycelia age was 6 d, mannitol was 0.5 mol, the No. 3 medium ( sugar 10 g, glucose 4 g, yeast power 4 g, mannitol 0.5 mol, pH value 6.2, agar 18 g, the total volume 1000 ml). Under the best conditions, the protoplast regeneration rate was 0.43%. [Conclusion] The optimum extracting procedure was simple, reasonable and reliable.关键词
冬虫夏草/原生质体/再生Key words
Cordyceps sinensis/ Protoplast/ Regeneration分类
农业科技引用本文复制引用
朱蕴兰,周卫东,陈安徽,陈宏伟,李超..冬虫夏草无性型原生质体再生条件研究[J].安徽农业科学,2009,37(30):14629-14631,3.基金项目
江苏省高校自然科学研究计划项目(05KJD180203) （05KJD180203）
江苏省"333高屡次人才培养工程"资助项目(2007,4). （2007,4）
