安徽农业科学2009，Vol.37Issue(30)：14725-14726,2.
鸡毒支原体灭活抗原超滤浓缩工艺的研究
Research on the Ultrafiltration and Concentration Technique for Inactivated Antigen of Mycoplisma gallisepticum
胡来根 1张小飞 1于漾 1尹秀凤 1薛家宾 1何家惠1
作者信息
- 1. 南京天邦生物科技有限公司,江苏,南京,211102
- 折叠
摘要
Abstract
[Objective] The aim was to establish a set of antigen concentration technology for producing inactivated vaccine of Mycoptasma gallisepticum. [Method] The ultrafiltration and concentration experiment was carried out on the inactivated antigens of M. gallisepticum with Millipore medium boxy ultrafiltration system and the absorbance changes of filtrates concentrated for different times were determined by UV spectrophotometry. [Result] There was no bacterial contamination in disinfectant solution after autoclave sterilization and there was no bacterial contamination in return fluid in ultrafiltration instrument after cycling, before and after antigen concentration. It was found after Wright' s staining that except that there was no mycoplasma in the sample of tail liquid, there were blue mycoplasroa thalli with irregular shapes appeared in the other samples. After concentration with 100 K ultrafiltration membrane stack, there was no mycoplasma antigen leaked. The absorbance of the solution was increased as the concentration times were increased. After the stock solution was concentrated for 5, 10 and 15 times, the absorbance was increased for 0.718, 1.281 5 and 1.464 5 resp. [Conclusion] The production process suitable for the cultrafiltration and concentration of M. gaiiisepticum angiten was established in this experiment and it laid a foundation for preparing M. gailisepticum vaccine and its multivalent vaccine.关键词
鸡毒支原体/超滤浓缩/抗原Key words
Mycoplasma gailisepticum/ Ultrafiltration and concentration/ Antigen
分类
农业科技引用本文复制引用
胡来根,张小飞,于漾,尹秀凤,薛家宾,何家惠..鸡毒支原体灭活抗原超滤浓缩工艺的研究[J].安徽农业科学,2009,37(30):14725-14726,2.
