安徽农业科学2009，Vol.37Issue(30)：14983-14986,14992,5.
新西伯利亚银白杨微枝试管外生根与微环境因子的关系
Relationship between Micro-environmental Factors and ex Vitro Rooting of Micro Cuttings of Populus bachofenii × P. pyramidalis ROZ
摘要
Abstract
[Objective] The purpose was to enhance the acclimation efficiency of ex vitro rooting of Populus bachofenii × P. pyramidalis ROZ. [Method] With the clonal tissue cultured micro cuttings of P. bachofenii × P. pyramidalis as materials, the correlation between micro-environmental factors and its ex vitro rooting rate and roots quality was studied through comparative experiment. [Result] The rooting effect of micro cuttings on the medium 25% peat + 30% vermiculite + 45% perlite was best and its subordinate function value, rooting rate, root number, root length and partial root rate were 0. 857, 90% ,11.6 per plant, 2.25 cm and 11 % resp. The rooting effect of micro cuttings treated with IBA was best and the subordinate function value, rooting rate, average root number, average root length and partial root rate were 0.785, 93.33% , 6.12 per plant, 1.15 cm and 13.67% resp. in the treatment with 300 mg/L IBA. The rooting effect of micro cuttings was best under 20 ℃ and its subordinate function value, rooting rate, root number, root length and partial root rate were 0.820, 100.00% , 7.80 per plant, 1. 17 cm and 15.33% resp. The rooting effect of micro cuttings treated with light intensity of 75 μmol/(m~2·s) was best and its subordinate function value, rooting rate, root number, root length and partial root rate were 0.686, 100.00% , 8.76 per plant, 2.08 cm and 38.00% resp. The rooting effect of micro cuttings on the medium with water content of 50% was best and its subordinate function value, rooting rate, root number, root length and partial root rate were 0.894, 100.00% , 7.73 per plant, 1.75 cm and 13. 33% resp. [Conclusion] The study laid a foundation for establishing the rapid and effective micro-propagation technical system of P. bachofenii × P. pyramidalis.关键词
新西伯利亚银白杨/组培微枝/试管外生根/微环境因子Key words
Populus bachofenii × P. pyramidalis ROZ/ Tissue-cultured micro cuttings/ Ex vitro rooting/ Micro-environmental factors分类
农业科技引用本文复制引用
梁立东,杨玲,沈海龙,刘春苹..新西伯利亚银白杨微枝试管外生根与微环境因子的关系[J].安徽农业科学,2009,37(30):14983-14986,14992,5.基金项目
国家林业局"948"引进项目(2004-4-03). （2004-4-03）
