安徽农业科学2009，Vol.37Issue(31)：15137-15138,2.
广西姜花花粉离体萌发培养基的筛选
Medium Selection for the Pollen Germination in Vitro of Hedychium kwangsiense T.L.Wu & S.J.Chen
董芬丽 1吴志 2胡秀3
作者信息
- 1. 中国科学院华南植物园,广东广州510650
- 2. 仲恺农业工程学院,广东广州,510225
- 3. 中国科学院研究生院,北京,100081
- 折叠
摘要
Abstract
South China Botanical Garden,Chinese Academy of Science,Guangzhou,Guangdong 510650)
[Objective] The aim was to screen out the optimum medium for the pollen germination in vitro of Hedychium kwangsiense T. L. Wu &S. J. Chen. [ Method] With situ-germination determination as CK.on the basis of orthogonal pre-experiment,the optimum medium for the pollen germination in vitro of H. kwangsiense was screened out through single factor experiments. [ Result ] When the sucrose concn. was 10.000% ,the germination rate in vitro of H. kwangsiense pollens was highest, being 22.45%. When the concn. of boric acid was 0.010% and 0. 050% ,the germination rates in vitro of H. kwangsiense pollens were 35. 58% and 37. 42% reap. When the concn. of calcium chloride was 0.01% ,the germination rate in vitro of H. kwangsiense pollens was 38.49%. In the concn. range of 0.010% -0.200% ,the germination rate in vitro of H. kwangsiense pollens was decreased as the concn. of calcium chloride was increased. The optimum medium for the pollen germination in vitro of H. kwangsiense was:10.000% sucrose + 0.050% boric acid + 0.010% calcium chloride +2.000% agar.its pH value was 7.0 and the culture temperature was 20℃. The germination rate of pollens determined through pollen germination in vitro on the selected medium was 46.00% and that determined by pollen situ-germination determination was 50.00%. [Conclusion] The pollen vitality of H. kwangsiense could be rapidly and accurately determined by using the selected medium.关键词
广西姜花/花粉/离体萌发/活力测定Key words
Hedychium kwangsiense T. L. Wu & S. J. Chen/ Pollen/ Germination in vitro/ Test on vitality分类
农业科技引用本文复制引用
董芬丽,吴志,胡秀..广西姜花花粉离体萌发培养基的筛选[J].安徽农业科学,2009,37(31):15137-15138,2.
