安徽农业科学2009，Vol.37Issue(31)：15141-15143,15156,4.
成熟石榴叶片DNA提取方法研究
Study on DNA Extraction Methods of Mature Pomegranate (Punica granatum Linn.) Leaves
摘要
Abstract
[Objective] The research aimed to extract the high quality and yield genomic DNA from mature pomegranate (Punica granatum Linn. ) leaves. [ Method ] According to the character of containing amylase and polyphenol, genomic ONA in fresh leaves, frozen leaves and dry leaves of 2 kinds of mature pomegranates were extracted by improved CTAB I and CTAB U method. And they were identified by agarose gel elec-trophoresis,ultraviolet spectrophotometer method and enzyme digestion. [Result] The results showed that the sample hole was clean and no pulling, OD_(260nm)/OD_(280nm) was 1.7-1.9 when the genomic DNA extracted by improved CTAB II method was electrophoresis. And its quality and yield was high than that extracted by improved CTAB I method. [ Conclusion] The improved CTAB H method was the effective method of extracting the high quality and yield genomic DNA from mature pomegranate (Punica granatum Linn. ) leaves.关键词
石榴/成熟叶片/CTAB法/基因组DNA/提取Key words
Pomegranate (Punka granatum Linn. ) / Mature leaves/ CTAB method/ Genomic DNA/ Extraction分类
农业科技引用本文复制引用
赵丽华,王先磊..成熟石榴叶片DNA提取方法研究[J].安徽农业科学,2009,37(31):15141-15143,15156,4.基金项目
西昌学院课题(5092) （5092）
会理县科技局(XA0605). （XA0605）
