安徽农业科学2009，Vol.37Issue(31)：15172-15174,15179,4.
羊草Class Ⅱ几丁质酶基因的克隆及序列分析
Molecular Cloning and Sequence Analysis of Class II Chitinase Gene in Leymus chinensis
摘要
Abstract
[Objective] The aim was to clone Class II chitinase gene in Leymus chinensis which are growing in saline land in Heilongjiang Province,and lay a foundation for further researching the biological function and application of chitinase gene. [ Method] Constructing cDNA library of Leymus ehinensis leaves, its DNA sequence was determined and analyzed, the homology of chitinase gene and amino acid sequence was compared with that in the GeneBank. [Result] The total length cDNA segment was cloned,the length was 996 bp.the ORF was 768 bp among which, encoding 255 amino acids. It had the structural character of Class II chitinase gene because the encoded products lacked CBD and C-temiinal extension region. The amino acid sequence had high homology compared with Class II chitinase gene of rye and wheat. The constructed recombinant vector pQE-LcChi2 can induce and express a protein of 27 KD, the result was consistent with deduced encoded product of pQE-Lc-Chi2 gene. [Conclusion] LcChi2 gene has expressed function,which can express in E. coli.关键词
羊草/几丁质酶基因/克隆/序列分析Key words
Leymus chinensis/ Chitinase gene/ Cloning/ Sequence analysis分类
农业科技引用本文复制引用
金华,安晓雯,姜国斌..羊草Class Ⅱ几丁质酶基因的克隆及序列分析[J].安徽农业科学,2009,37(31):15172-15174,15179,4.基金项目
辽宁省教育厅科学技术研究项目(2008120)和大连民族学院引进人才启动基金(20056209)资助. （2008120）
