安徽农业科学2009，Vol.37Issue(31)：15175-15176,15182,3.
牛瑟氏泰勒虫18S rRNA基因的克隆及分子分类学分析
Ooning and Molecular Taxonomy Analysis of 18S rRNA Gene of Cattle Theileria sergenti
摘要
Abstract
To analyze 18S rRNA nucleotide sequence of cattle Theileria sergenti, two pairs of specific primers were deigned according to 18S rRNA gene of cattle Theileria sergenti sequences published on CenBank. 18S rRNA gene was amplified by PCR and cloned into pMD18-T vector. Positive clones were identified by PCR screening and restriction digestion enzyme. The phylogenetic tree was inferred based on 18S rRNA sequence of Yanbian and the other eight species of Theileria available on GenBank. Sequencing of positive clones showed that the cloned gene has a total length of 1 744 bp. The phylogenetic tree indicated that Yanbian strain, Lanzhou strain, Japan strain of cattle T. sergenti were closer to China strain of T. buffili,but Yanbian strain was far from T. mutatis.关键词
牛瑟氏泰勒虫/18SrRNA基因/克隆/分子分类学Key words
Cattle Theileria sergenti/ 18S rRNA gene/ Cloning/ Molecular taxonomy分类
农业科技引用本文复制引用
沈岩,许应天,李静,栾杨,杨兴..牛瑟氏泰勒虫18S rRNA基因的克隆及分子分类学分析[J].安徽农业科学,2009,37(31):15175-15176,15182,3.基金项目
吉林省科技发展计划项目(20050703-4). （20050703-4）
