安徽农业科学2009，Vol.37Issue(32)：15661-15664,15700,5.
低温污水处理器中耐冷菌脱氢酶活性分析
Analysis on the Dehydrogenase Activity in Cold-resistant Bacteria in the Processor of Low-temperature Wastewater
姜明 1郭立萍 1曲艳娇 1曹振岭 1韩晓云2
作者信息
- 1. 牡丹江师范学院生物系,黑龙江牡丹江,157012
- 2. 黑龙江大学生命科学学院,黑龙江哈尔滨,150080
- 折叠
摘要
Abstract
[Objective] The aim was to supply technical support for solving the problem that the sewage was difficult to dispose in north China in winter. [Method] The influences of pH value, temperature and time on the dehydrogenase activity in 8 strains of cold-resistant bacteria isolated, purified and identified from low-temperature biofilm were analyzed by TTC method. [Result] The optimum pH value of the strains S1, S4, S5, S7 and S8 at both optimum temperature and 4 ℃ was 7.5 and that of the other 3 strains at the 2 temperatures were different, but they were in the range of 7.5-8.5. The optimum reaction temperature of the strains S1, S3, S4, S5 and S6 was 30 ℃ and that of the strains S2, S7 and S8 was 20 ℃. Except for the strain S3, the optimum reaction time of the other strains at 4 ℃ was longer than their optimum reaction time at optimum reaction temperature. At 4 ℃, the optimum pH values of the strains S3, S4 and S6 were 8.5, 7.5 and 8.0 resp., their optimum reaction time were 0.6, 12.0 and 0.5 h resp. and their highest dehydrogenase activities were 7.19, 6.73 and 7.70 mg(TF)/g(SS) resp. [Conclusion] The dehydrogenase activities of the strains S3, S4 and S6 were higher and they were suitable to be used in processing low-temperature sewage.关键词
低温生物膜/TTC-脱氢酶/耐冷菌/微生物活性Key words
Low-temperature biofilm/ TTC-dehydrogenase/ Cold-resistant bacteria/ Microbial activity分类
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姜明,郭立萍,曲艳娇,曹振岭,韩晓云..低温污水处理器中耐冷菌脱氢酶活性分析[J].安徽农业科学,2009,37(32):15661-15664,15700,5.
