安徽农业科学2009，Vol.37Issue(32)：15971-15973,3.
根霉Yc1发酵豆渣产纤溶酶的研究
Study on Fermentation Conditions of Fibrinolytic Enzyme Produced by Rhizopus sp. Yc1 Soy Residue
摘要
Abstract
[Objective]The optimization of liquid state fermentation conditions of fibrinolytic enzyme produced by Rhizopus sp. Yc1 from soy residue were studied. [Method]The optimum fermentation conditions were determined by monofactor test and orthogonal design . [Result] The optimum medium consisted of wheat bran 5.0%,soy residue flour 3.0%,ZnCl_2 0.03%,MgSO_4 0.15%,Na_2HPO_4 0.05%;and the medium initial pH 7.0, 60 ml medium in 250 ml Erlenmeyer,rotation speed 180r/min for 60h at 32 ℃.The fibrinolytic enzyme productivity reached 176.2 U/ml on optimized condition,which is more than 92.3 U/ml that of the original conditions.[Conclusion]The fibrinolytic enzyme produced from soy residue would be a safety cellosolve,which with high activity, low side reaction and low cost.The study provides soy residue as produce material with a new direction.关键词
根霉/纤溶酶/豆渣/发酵条件Key words
Rhizopus sp./ Fibrinolytic enzyme/ Soy residue/ Fermentation condition分类
农业科技引用本文复制引用
朱萍,严萍,周河治..根霉Yc1发酵豆渣产纤溶酶的研究[J].安徽农业科学,2009,37(32):15971-15973,3.基金项目
广西大学科研基金资助项目(X081026). （X081026）
