安徽农业科学2009，Vol.37Issue(32)：15703-15705,15708,4.
多花胡枝子再生体系初探
Research in the Regeneration System of Lespedeza floribunda Bunge.
摘要
Abstract
[Objective] The aim was to study regeneration system of Lepedeza floribunda. [Method] Cotyledonary node was adopted as primary differentiation explant to induce adventive buds. Regenerated plants were obtained after rooting culture of shoots which acquired in secondary differentiation. [Result] The optimum time of seed disinfection was 0.1% HgCl_2 solution disinfected 8 min. Primary differentiation acquired cotyledonary nodes as explant and the optimal prescription was half strength MS medium plus 6-BA 1.0 mg/L, NAA 0.05 mg/L,and IBA 0.2 mg/L. The suitable medim of secondary differentiation for propagated shoots was WS medium containing 6-BA 0.7 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L. The efficient rooting prescription was WS medium with IBA 0.3 mg/L and rooting ratio was 74.8%. The effect of 6-benzylaminopurine (6-BA ) in primary differentiation reached the significant level (α=0.05) even secondary differentiation. Indole-3-butyric acid (IBA),better than Napthaleneacetic acid (NAA), was able to induce regenerated shoot rooting.关键词
多花胡枝子/子叶节/再生体系Key words
Lespedeza floribunda/ Cotyladonary node/ Regeneration system分类
农业科技引用本文复制引用
卢迪,杨晓红,刘雯,常默,王晓霜..多花胡枝子再生体系初探[J].安徽农业科学,2009,37(32):15703-15705,15708,4.基金项目
北京市教委项目(KM20071002007). （KM20071002007）
