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楸树组培与快繁技术初探

周蓉 谢焕松 刘鑫燕 曹云英

安徽农业科学2009,Vol.37Issue(32):15715-15716,15794,3.
安徽农业科学2009,Vol.37Issue(32):15715-15716,15794,3.

楸树组培与快繁技术初探

Preliminary Study on Tissue Culture and Fast Propagation Technology of Catalpa bungei

周蓉 1谢焕松 1刘鑫燕 1曹云英1

作者信息

  • 1. 南通大学生命科学学院,江苏南通,226007
  • 折叠

摘要

Abstract

[Objective] The aim was to achieve rapid propagation of Catalpa bungei and provide a theoretical basis for large-scale seedling production. [Method] Axillary bud of Catalpa bungei were used as explants, while different medium and hormone combinations were selected to set up the tissue culture and rapid propagation system of Catalpa bungei . [Result] N_6+2.0 mg/L 6-BA +0.005 mg/L NAA is an appropriate culture medium for germination of axillary bud, MS+1.5 mg/L 6-BA +2.0 mg/L NAA is the best medium for callus induction, MS+0.5 mg/L 6-BA +0.5 mg/L NAA is suitable for bud differentiatation and N_6+1.5 mg/L 6-BA +0.005 mg/L NAA for subculture multiplication. 1/2 MS+0.5 mg/L NAA was of advantage to rooting culture. [Conclusion] The propagation speed of Catalpa bungei was greatly improved by tissue culture method.

关键词

楸树/组织培养/腋芽/外植体/愈伤组织

Key words

Catalpa bungei/ Tissue culture/ Axillary bud/ Explants/ Callus

分类

农业科技

引用本文复制引用

周蓉,谢焕松,刘鑫燕,曹云英..楸树组培与快繁技术初探[J].安徽农业科学,2009,37(32):15715-15716,15794,3.

基金项目

南通大学校风课题(06Z108)和南通大学教学研究课题资助. (06Z108)

安徽农业科学

OA北大核心CSTPCD

0517-6611

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