安徽农业科学2009，Vol.37Issue(33)：16213-16215,3.
棘豆蠕孢菌发酵液中次级活性产物分离纯化初探
Preliminary Study on the Isolation and Purification of Active Secondary Metabolites in the Fermentation Broth of Embellisia oxytropis
摘要
Abstract
[Objective] The study aimed to lay a theoretical foundation for seeking for endophytic fungi with capability of synthesizing the same or similar active components which was synthesized in their host plants. [Method] The mycelia and fermentation broth were isolated by pumping filtration with Buchner funnel, the impure proteins, pigments, multivalent metal ions and polysaccharides were scavenged from fermentation broth through pretreatments. Then the fermentation products were isolated with macroporous resin and purified by silica gel chromatography. Finally, the sample and the component from host plants were ascertained to belong to the same class of alkaloid by infrared spectrum detection. [Result] The improved bismuth potassium iodide made sample show as a tangerine spot and the Ehrlich's chromogenic reagent made it show as a purplish red spot. Both of their Rf values were 0.78. The reactions of the water elution products and the 3 kinds of precipitants were negative. The reaction of alcohol-water elution products and bismuth potassium iodide was negative and that of alcohol-water elution products and picric acid and tannic acid were positive. The reactions of alcohol elution products and the 3 kinds of precipitants were positive. It was shown in the identification results that the isolated sample was hydroxyl substituted indolizidine alkaloids. [Conclusion] The endophytic fungus Embellisia oxytropis had the capability of synthesizing the same or similar active components which was synthesized in its host plant Gansu whin.关键词
棘豆蠕孢菌/分离纯化/生物碱Key words
Embellisia oxytropis/ Isolation and purification/ Alkaloid分类
农业科技引用本文复制引用
时东方,李丽,郑梅竹,李广洲,张红晶,王琦..棘豆蠕孢菌发酵液中次级活性产物分离纯化初探[J].安徽农业科学,2009,37(33):16213-16215,3.基金项目
国家自然科学基金项目(30970554). （30970554）
