安徽农业科学2009，Vol.37Issue(33)：16246-16249,4.
枯草杆菌重组水蛭素(HV3)的发酵工艺研究
Study on Fermentation Process for Bacillus subtilis Producing Recombining Hirudin (HV3)
摘要
Abstract
[Objective] The study was to determine the optimal fermentation process for Bacillus subtilis producing hirudin. [Method] With B. subtilis as the tested strain, the effects of activation time of strain, inoculation quantity, medium volume, temperature, initial pH value, carbon resource and nitrogen resource on fermentation were studied. And on the basis of that, the fermentation condition was optimized. [Result] The optimal fermentation condition was that temperature of 37 ℃, initial pH value of 7, medium volume of 20 ml /250 ml, activation time of strain of 6 h, inoculation quantity of 3%, nitrogen resource of 1.5% Tryptone, carbon resource of 9.0% soybean leaching solution, rotation rate of 220 r/min, fermentation time of 29 h and 0.3%Tween 80 was added after 16 h of fermentation. [Conclusion] Under the optimal condition, the activity of fermentation liquid could reach 210 ATU/ml, increasing 13.1 times than that before optimization. When the optimal fermentation condition was used in 50 L enlarged tank, the repeatability of test results was good, and the activity of fermentation liquid could reach 208 ATU/ml.关键词
水蛭素/枯草杆菌/发酵Key words
Hirudin/ Bacillus subtilis/ Fermentation分类
农业科技引用本文复制引用
陈华友,齐向辉,谭小力,乌慧玲..枯草杆菌重组水蛭素(HV3)的发酵工艺研究[J].安徽农业科学,2009,37(33):16246-16249,4.基金项目
国家"863"项目(2006AA03Z0453) （2006AA03Z0453）
江苏省高校自然科学研究项目(09KJB230001) （09KJB230001）
江苏大学校基金项目(08jdg011) （08jdg011）
江苏省博士后科研项目(0901012B). （0901012B）
