安徽农业科学2009，Vol.37Issue(33)：16280-16281,2.
胞外表达L-天冬酰胺酶发酵条件的研究
Study on the Fermentation Conditions of Extracellular Expression L-asparaginase
摘要
Abstract
[Objective] The research aimed to optimize the fermentation conditions of the engineering bacteria that secreted L-asparaginase into media. [Method] The ingredients of media were studied by orthogonal design. The effects of inducing conductions and aeration flow on enzyme production were studied by single-factor design. [Result] Fermentation media was composed of 8.0% corn steep liquor and 1.0% glycerol. 0.5 g/L lactose was added into media after culture 10 h to induce protein expression for 12 h. An appropriate aeration flow was 0.83 VVM. [Conclusion] This research provided basis for industrial production of extracellular expression L-asparaginase.关键词
L-天冬酰胺酶/胞外表达/发酵Key words
L-asparaginase/Extracellular expression/ Fermentation分类
农业科技引用本文复制引用
王利群,黄达明,赵希岳,王文兵,徐苑..胞外表达L-天冬酰胺酶发酵条件的研究[J].安徽农业科学,2009,37(33):16280-16281,2.基金项目
常州市科技局项目(CS2006003) （CS2006003）
镇江市科技局项目(CY2006018). （CY2006018）
