安徽农业科学2009,Vol.37Issue(33):16253-16254,16260,3.
牡丹组织培养
Peony Tissue Cultivation
纪庆亮 1蒋素华 2秦仪利3
作者信息
- 1. 南京林业大学森林资源与环境学院,江苏南京,210037
- 2. 河南农业大学生命科学院,河南郑州,450002
- 3. 河南省鹤壁市培红高中,河南鹤壁458030
- 折叠
摘要
Abstract
[Objective]The research aimed to study the techniques of isolated culture of peony to implement rapid propagation.[Method]Take MS as the basic culture medium,in vitro culture of peony's embryo was carried on by adding different concentration of NAA, BA and IAA.[Result]Grow of vitro embryo was similar to the grow pattern of embryo when it was germinating on the culture medium which has no hormone, cotyledon expanded and grown, and stopped growing when it achieved approximately seed size; cotyledon growth was improved by BA, the growth of root was inhibitted when consdntration of BA increased; NAA could improve the fromation of callus and inhibit the growth of root. Growth of hypocotyl and root of peony embryo were improved by 0.1 mg/L BA, which was conducive to the fromation of seedlings.[Conclusion]It was provided the feasible way for expanding propagation of peony.关键词
牡丹/培养基/胚培养Key words
peony/culture medium/embrionic raise分类
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纪庆亮,蒋素华,秦仪利..牡丹组织培养[J].安徽农业科学,2009,37(33):16253-16254,16260,3.