安徽农业科学2009，Vol.37Issue(33)：16261-16262,16270,3.
延边黄牛GHR基因第8外显子一个新多态位点的发现
Discovery on a New Polymorphic Site of GHR Gene Exon 8 in Yanbian Cattle
摘要
Abstract
[Objective] The study aimed to seek for molecular marker used in the marker aid selection and provide the basis for molecular breeding of Yanbian cattle. [Method] With the blood samples from 46 pure breeds of Yanbian cattle as tested materials, the exon 8 of GHR gene was detected and sequenced by using PCR-SSCP technique and the genetic variation of GHR in Yanbian cattle population was determined. [Result] SSCP detection on amplified product discovered tha there were 3 genotypes as AA, BB and AB, in which, AA and BB were homozygous and AB was heterozygous. The sequencing analysis with AA and BB as the individuals discovered that there was a new polymorphic site. The genetic analysis on the polymorphic site of GHR gene showed that AA individuals were more and AB and BB individuals were less. AA genotype effect was superior to that of other genotypes on some growth traits, with the difference being significant among genotypes. [Conclusion] This study suggested that GHR gene may be a candidate gene responsible for growth trait in Yanbian cattle and more gene sites should be combined in breeding so as to realize the effective breeding.关键词
延边黄牛/GHR基因/PCR-SSCP/多态性Key words
Yanbian cattle/ GHR gene/ PCR-SSCP/ Polymorphism分类
农业科技引用本文复制引用
蔡锦顺,张嘉保,严昌国,姜昊,高岩,梁成云..延边黄牛GHR基因第8外显子一个新多态位点的发现[J].安徽农业科学,2009,37(33):16261-16262,16270,3.基金项目
国家科技支撑计划项目(2007BAD55B03). （2007BAD55B03）
