北京师范大学学报（自然科学版）2009，Vol.45Issue(3)：284-289,6.
黄河水体多环芳烃降解菌的筛选及其降解性能研究
ISOLATION AND CHARACTERIZATION OF PAHS-DEGRADING BACTERISA FROM THE YELLOW RIVER
摘要
Abstract
Three indigenous PAHs-degrading bacterial strains HKS-1, HKS-2 and HKS-3 were obtained by selective culture from The Yellow River. Sequence analysis of 16 s rDNA showed that HSK-1 belongs to Bacterium sp. , HSK-2 and HSK-3 belongs to Bacillus sp. Biodegradation experiments showed that PAHs degradation in water-sediment systems could be fitted with zero-order kinetics (the first 25 days were fitted). These microbial strains degraded chrysene and benzo[a]pyrene well, the presence of particulates promoted degradation. With the initial concentrations of chrysene and benzo[a]pyrene in water/sediment systems at 15. 85 and 12. 10 μg·L~(-1), 45. 79% and 37. 36% of chrysene and benzo[a]pyrene respectively were degraded after 35 d incubation without sediment; at the same time, 58. 76% and 51. 69% of chrysene and benzo[a]pyrene respectively in the system were degraded with sediment. Water and PAHs can cross PTFE membrane but bacteria and sediment cannot, a membrane experiment was carried out to compare the biodegradation rates of PAHs in water phase and in particulate phase. It was found that when zero-order biodegradation kinetics was fitted (the first 4 days), degradation rate for chrysene and benzo[a]pyrene at the particulate phase were three and four times of that in water phase, respectively.关键词
多环芳烃降解菌/泥沙/黄河Key words
PAHs-degrader/ sediment/ Yellow River分类
天文与地球科学引用本文复制引用
王然,夏星辉,李恭臣,周追..黄河水体多环芳烃降解菌的筛选及其降解性能研究[J].北京师范大学学报（自然科学版）,2009,45(3):284-289,6.基金项目
国家自然科学基金资助项目(40871228) （40871228）
