北京师范大学学报（自然科学版）2009，Vol.45Issue(3)：259-262,4.
关于(A6,A11)-双位点突变胰岛素原作为外源肽呈递骨架可行性的研究
(A6, All)-MUTANT PROINSULIN AS A SCAFFOLD FOR RESENTING EXOGENOUS FUNCTIONAL-MOTIFS
摘要
Abstract
A molecular chimera was constructed on the scaffold of (A6, All)-proinsulin by introducing an anti-thrombosis motif between the B29 and A2 sites. The chimera gene was expressed in Escherichia coli. The expressed product was purified, its biological activities determined for insulin receptor binding, insulin immunoactivity, and anti-platelet aggregation. It was found that the chimera peptide had potent anti-platelet activity but was without insulin activity. These data indicate that the (A6, All)-proinsulin can be used as a scaffold for presenting exogenous functional motifs.关键词
(A6,A11)-双位点突变人胰岛素原/外源抗栓肽/呈递骨架Key words
(A6,A11) proinsulin/ anti-thrombosis motif/ presentating scaffold分类
医药卫生引用本文复制引用
井健,唐建国..关于(A6,A11)-双位点突变胰岛素原作为外源肽呈递骨架可行性的研究[J].北京师范大学学报（自然科学版）,2009,45(3):259-262,4.基金项目
国家自然科学基金面上资助项目(30540071) （30540071）
