北京中医药大学学报2009，Vol.32Issue(9)：624-625,645,3.
可见分光光度法测定玉簪花中总皂苷的含量
Quantitative determination of the total saponins from Flos Hostae Plantagineae by ultraviolet-visible spectrophotometry
摘要
Abstract
Objective To set up a ultraviolet-visible spectrophotometry for quantitative determination of the total saponins from Flos Hostae Plantagineae. Methods The ultraviolet-visible spectrophotometry was used to detect the absorbance with the wavelength of 450 nm taking vanillin-glacial acetic acid-concentrated sulphuric acid as the color-developing agent and gitogenin as the control sample. The content of the total saponins in the samples was calculated by using standard curve. Results Gitogenin showed a good linear relationship at range of 14～70 μg/ml (r=0.999 2) with the average recovery of 96.8% (RSD=2.97%, n=6). Conclusion The method is easy, accurate and sensitive with a higher repeatability, which can be used in the quantitative determination of the total saponins from Flos Hostae Plantagineae.关键词
玉簪花/支脱皂苷元/总皂苷/可见分光光度法/含量测定Key words
Flos Hostae Plantagineae/ gitogenin/ total saponins/ visible spectrophotometry/ quantitative determination分类
医药卫生引用本文复制引用
解红霞,薛培凤,张宏桂,樊丽清,边红梅..可见分光光度法测定玉簪花中总皂苷的含量[J].北京中医药大学学报,2009,32(9):624-625,645,3.基金项目
2008年内蒙古自治区人才开发基金项目 （）
