北京中医药大学学报2009，Vol.32Issue(9)：626-629,4.
葛根中葛根黄酮的水提取及大孔树脂纯化工艺研究
Optimization of water extraction and macroporous resin purification processes of flavonoids from Radix Puerariae
摘要
Abstract
Objective To optimize the processes of water extraction and macroporous resin purification of flavonoids from Radix Pueraiae. Methods The orthogonal test was used to optimize the water extraction process and the monofactorial observation to optimize the macroporous resin purification process, with the extraction rates of puerarin and the total flavonoids as the indexes. Results The optimum water extraction process was as follows: 9 times mount of water was used to extract pueraria for 3 times with 90 min each time. The extraction rate of the total flavonoids was 92.17% and that of puerarin, 84.91%. The optimum macroporous resin purification process was as follows: macroporous resin HPD-750 was used with the ratio of diameter to length as 1:6, the concentration of the sample solution was 1.5 g/mL, the maximum sample was 3 BV, the flowing rate was 1 mL/min, the eluant was 70% ethanol and the eluant volume was 5 BV. The extract yield was 4.47% after macroporous resin purification. The content of total flavonoids was 76.22% and that of puerarin, 56.20%. Conclusion This optimum process is easy to operate, which can be applied in large-scale producution of the total flavonoids and puerarin.关键词
葛根黄酮/大孔树脂/提取/纯化Key words
pueraria flavones/ macroporous resin/ extract/ purification分类
医药卫生引用本文复制引用
李旭洲,韩立炜,张岩峰,倪健..葛根中葛根黄酮的水提取及大孔树脂纯化工艺研究[J].北京中医药大学学报,2009,32(9):626-629,4.基金项目
国家自然科学基金项目(No.30400601) （No.30400601）
