北京中医药大学学报2009，Vol.32Issue(10)：683-685,彩页1,4.
白藜芦醇影响OVCAR3细胞生长并通过活化caspase-3诱导细胞凋亡的研究
Influence of resveratrol on growth of OVCAR3 cells and its inducing effect on apoptosis through activating caspase-3
摘要
Abstract
Objective To discuss the influence of resveratrol on the growth and cell cycle of human ovarian cancer cells ( OVCAR3), and to study its inducing effect on the apoptosis of OVCAR3 and the possible pathway. Methods The influences of resveratrol on the growth, karyopycnosis and stegmentization were detected or observed by applying respectively MTT test, fluorescence microscope after Ho33342 staining, and the apoptosis of OVCAR3 was detected by using TUNEL. The flow cytometry was used to analyze the effects of resveratrol on cell cycle distribution and activity of caspase-3. Results The proliferation of OVCAR3 was promoted by resveratrol in low concentration ( <30 μmol/L) and inhibited in high concentration ( > 30 μmol/L). The retardation of S/G_2 cells and OVCAR3 apoptosis could be induced by resveratrol. The increase of caspase-3 activity induced by resveratrol showed a time-efficiency and concentration-efficiency. Conclusion Resveratrol can improve or inhibit the growth of OVCAR3 in a manner of concentration dependency, and it can induce OVCAR3 apoptosis through promoting caspase-3 activity.关键词
白藜芦醇/细胞凋亡/caspase-3Key words
resveratrol/ cell apoptosis/ caspase-3分类
医药卫生引用本文复制引用
黄春芳,牛建昭,王继峰,赵丕文..白藜芦醇影响OVCAR3细胞生长并通过活化caspase-3诱导细胞凋亡的研究[J].北京中医药大学学报,2009,32(10):683-685,彩页1,4.基金项目
科技部国际科技合作计划资助项目(No.2008DFA319704,No.2006DFA31230),教育部国家外专局高等学科创新引智计划资助项目(No.1307007) （No.2008DFA319704,No.2006DFA31230）
