大豆科学2009，Vol.28Issue(4)：600-604,5.
一种适合大豆MicroRNA鉴定的RT-PCR方法
A RT-PCR Method Suitable for MicroRNA Identification of Soybean
摘要
Abstract
The microRNAs (miRNAs) are a newly identified class of small non-coding RNA,which regulate post-transcrip-tional gene expressing in eukaryote. Most miRNAs are predicted through the computer, the presentence/absence and the spatial/temporal of miRNAs still unclear. It is important for the establishment of efficient, sensitive and specific detection methods to detect miRNAs in different organism or period. In this study,the protocol derived from Lau et al. and Shi et al. was used to detect miR156a,predicted and released by Sanger Institute,in old or young leaf,root,and shoot for verifying this method. By comparison,this method is low cost, simple,fast, and sensitive, common for primer of RT-PCR, which could i-dentify a number of miRNAs in the same organism and compare miRNAs from different organism. Furthermore, this method need less RNA,in comparison with Northern Blot and Microarray.关键词
大豆/MicroRNA/RT-PCR/鉴定Key words
Soybean/ MicroRNA/ RT- PCR/ Identification分类
农业科技引用本文复制引用
刘永鑫,韩英鹏,常玮,邹权,郭茂祖,李文滨..一种适合大豆MicroRNA鉴定的RT-PCR方法[J].大豆科学,2009,28(4):600-604,5.基金项目
国家高技术研究发展计划(863计划)目标导向资助项目(2006AA10ZF1Z1) （863计划）
国家高技术研究发展计划(863计划)重点资助项目(2006AA100104-4). （863计划）
