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大豆叶片异黄酮含量与PAL基因相对表达量的关系

张大勇 李文滨 李冬梅 李永光

大豆科学2009,Vol.28Issue(4):670-673,4.
大豆科学2009,Vol.28Issue(4):670-673,4.

大豆叶片异黄酮含量与PAL基因相对表达量的关系

Relationship between the Contents of Soybean Isoflavanones and Relative Expression Quantity of PAL Gene

张大勇 1李文滨 1李冬梅 1李永光1

作者信息

  • 1. 东北农业大学大豆所,黑龙江,哈尔滨,150030
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摘要

Abstract

The phenylalanine ammonialyase( PAL) is an access point enzyme and also one of isoflavone synthesis key enzyme in the phenylalanine pathways. In order to study the relationship between the contents of soybean isoflavanones and relative expression quantity of PAL gene, a sand culture experiment was conducted. Soybean leaves at different growth stage were sampled, and the isoflavone content were measured and the relative expression quantity of alanin ammonialyase ( PAL) gene were determined by RQ- PCR. There was a coordination fluctuation tendency between phenylalanin ammonialyase ( PAL) gene relative expression quantity and isoflavone content in soybean leaf. Soybean isoflavones content and relative expression quantity of phenylalanin ammonialyase( PAL) gene appeared a peak value at Rl stage.

关键词

大豆异黄酮/苯丙氨酸解氨酶/相对表达量

Key words

Isoflavones/ PAL/ Relative expression quantity

分类

农业科技

引用本文复制引用

张大勇,李文滨,李冬梅,李永光..大豆叶片异黄酮含量与PAL基因相对表达量的关系[J].大豆科学,2009,28(4):670-673,4.

基金项目

国家科技支撑计划资助项目(2006BAD01A04) (2006BAD01A04)

黑龙江"十一五"科技攻关资助项目(GA068101-1-3). (GA068101-1-3)

大豆科学

OA北大核心CSCDCSTPCD

1000-9841

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