大豆科学2009，Vol.28Issue(4)：706-710,5.
改良环介导等温扩增技术快速检测转基因大豆
Development of a Loop-mediated Isothermal Amplification Assay for Rapid Detection of Transgenic Soybean
柳毅 1张军方 1张会彦 1苏旭东 1马晓燕 1亢春雨 1王雪静 1张伟1
作者信息
- 1. 河北农业大学食品科技学院,河北,保定,071001
- 折叠
摘要
Abstract
A loop-mediated isothermal amplification( LAMP) technology was established to detect transgenic soybean. The sequence of exogenous gene CP4 — EPSPS was used as target sequence, to design outer primers, inner primers and loop primers. The DNA extraction method was improved. Through visual observation of white precipitate to judge results of detection. The improved CTAB method isolated total DNA quickly and reducing DNA- extraction cost as compared with the traditional CTAB method. The detection limit of the genetically modified soybeans by LAMP technology was 0. 01% which was superior to PCR. Results indicate that LAMP can provide a sensitive, rapid yet simple test for the detection of CP4- EPSPS in transgenic soybean.关键词
环介导等温扩增/检测/CP4-EPSPS/转基因大豆Key words
Loop-mediated isothermal amplification/Detection/CP4- EPSPS/Transgenic soybean分类
农业科技引用本文复制引用
柳毅,张军方,张会彦,苏旭东,马晓燕,亢春雨,王雪静,张伟..改良环介导等温扩增技术快速检测转基因大豆[J].大豆科学,2009,28(4):706-710,5.
