大豆科学2009，Vol.28Issue(4)：566-573,8.
中国野生和栽培大豆蛋白质及油脂含量的比较分析
A Comparative Analysis of Protein and Fat Content between Wild and Cultivated Soybeans in China
摘要
Abstract
It is a basic work to explore the genetic variability and selection potential of protein and fat content existed in soybean germplasm for the improvement of the two components. In the present study,total 768 accessions,including 138 wild entries (Glycine soja Sieb. et Zucc.) ,408 cultivated landraces (Glycine max(L.) Merr.)and 145 released domestic culti-vars sampled from various eco- regions as a representative in China, along with 77 released foreign cultivars, were tested for their protein and fat content by using Kjeltec and Soxtec technique in order to characterize the genetic variability of protein and fat content in respective kinds of germplasm. The data obtained were analyzed with the software of SAS 9. 0 and SPSS 13. 0. The results obtaind under the environmental conditions in Nanjing showed that the range of protein content,fat content and total of both in wild soybean were 39. 2-54. 2%,7. 5- 17. 5%,47. 3-64. 6%, those in cultivated landraces 38. 8-51. 5%, 11. 5-22.5%,55.6-69.0%,those in released domestic cultivars 41. 7-49. 4%, 12. 9-22. 9%,55. 6-68. 6%,respectively. The increase of fat content and protein plus fat content after domestication and the decrease of protein content as well as its range implied that the improvement of fat content happened during the artificial improvement process. There existed large amount of genetic variation of protein content, fat content and total of both contents in various eco- regions in different kinds of materials,with elite accessions in each eco-regions,but the variation was not large enough among eco-region means. No significant correlation between the three contents and the original latitude of the materials was found in wild accessions, while significant positive correlation between fat content and latitude existed in cultivated landraces and released cultivars and significant negative correlation between protein content and latitude was found only in released cultivars. There was no significant correlation between protein content and fat content in wild soybeans, but significant negative correlation between the two contents found in cultivated land races and released cultivars. The reason for this kind of change should be the differential artificial selection among the eco-regions.关键词
野生大豆(Glycine soja Sieb.et Zucc.)/栽培大豆(Glycine max(L.)Merr.)/蛋白质含量/油脂含量/遗传变异/生态区域Key words
Wild soybean(Glycine soja Sieb. et Zucc.) /Cultivated soybean(Glycine max(L. )Merr.) /Protein content/Fat content/Genetic variability/Eco-region分类
农业科技引用本文复制引用
刘顺湖,周瑞宝,盖钧镒..中国野生和栽培大豆蛋白质及油脂含量的比较分析[J].大豆科学,2009,28(4):566-573,8.基金项目
国家重点基础研究发展规划资助项目(2004CB7206,2006CB101708,2009CB118404) （2004CB7206,2006CB101708,2009CB118404）
国家自然科学基金资助项目(30671266) （30671266）
国家高新技术研究发展计划资助项目(2006AA100104) （2006AA100104）
教育部高等学校创新引智计划资助项目(B08025) （B08025）
农业部公益性行业专项资助项目(200803060). （200803060）
