南方医科大学学报2009，Vol.29Issue(10)：2033-2036,4.
低温等离子体对细菌芽孢的灭活研究
Inactivation of bacterial spores using low-temperature plasma
摘要
Abstract
Objective To investigate the effect of low-temperature plasma on inactivation of bacterial spores and explore the mechanism. Methods Dielectric barrier discharge (DBD) was employed to generate the atmospheric low-temperature plasma for treatment of B.subtilis var. niger spores with the gas spacing of 3,4 and 5 and treatment time intervals of 5, 10, 15, 20, 25, 30 and 35 s. The survived colonies was counted with plate counting method, and the killing log value (KLV) at different treatment times was calculated. The inactivation effect of electric field on B.subtilis var.niger spores was also investigated and the spores treated with low-temperature plasma were observed with transmission electron microscope. Results With the gap spacing of 3, 4 and 5 mm, the KLV of low-temperature plasma on B.subtUis var.niger spores within 25, 30 and 35 s of exposure was more than 5. The germicidal effects of the electric field on B. subtilis var.niger spores were rather poor. Transmission electron microscopy demonstrated total destruction of the surface and interior structure of the spores by low-temperature plasma. Conclusions Low-temperature plasma is effective for inactivation of the bacterial spores with a time and dose dependence. The penetrating effect of charged particles and oxygenation effect of the reactive oxygen species might play a dominant role in plasma-induced bacterial spore inactivation, while the role of electric field is negligible.关键词
低温等离子体/枯草杆菌黑色变种芽孢/灭活效果/灭活机制Key words
Low-temperature plasma/ B.subtilis var.niger spores/ inactivation effect/ inactivation mechanism分类
医药卫生引用本文复制引用
石兴民,张冠军,袁育康,马跃,许桂敏,顾宁..低温等离子体对细菌芽孢的灭活研究[J].南方医科大学学报,2009,29(10):2033-2036,4.基金项目
国家优秀博士论文基金(200338) （200338）
新世纪优秀人才支持计划项目(NCET-04-0943),西安市科技创新支撑计划(YF07167) （NCET-04-0943）
