南方医科大学学报2009，Vol.29Issue(10)：2051-2054,4.
TLR2在大鼠穿透性角膜移植术后免疫排斥反应中的作用
Role of Toll-like receptor 2 in corneal graft rejection following penetrating keratoplasty
白浪 1陆晓和 2钟彦彦 2周瑾 2张静 2武海军2
作者信息
- 1. 南方医科大学南方医院眼科,广东,广州,510515
- 2. 南方医科大学珠江医院眼科,广东,广州,510282
- 折叠
摘要
Abstract
Objective To gain insight into the role of Toll-like receptor 2 (TLR2) in graft rejection following penetrating keratoplasty, and investigate the expression of TLR2 mRNA in the corneal graft. Methods Penetrating keratoplasty was performed in 3 groups of rats for orthotopic autologous corneal transplantation (group A), allograft corneal transplantation (group B), or allograft corneal transplantation with hormone treatment (C). The transparency and neovascularization of the cornea were observed using a slit-lamp microscope and scored according to the rejection index, with normal cornea serving as the control. The corneal tissues were sampled at 5, 7, and 9 days after the transplantation for histopathological examination and detection of TLR2 mRNA expression using RT-PCR. Results With the passage of time, edema, opacities and neovascularization of the comeal graft occurred after the operation in all the groups. Seven days after the operation, the rejection index of group B, but not that of groups A and C, met the diagnostic criteria for graft rejection with also support by histopathological evidence. The expression of TLR2 mRNA was detected in normal corneas and augmented in the corneal grafts in the 3 transplantation groups. TLR2 mRNA expression in group B was significantly higher than that of group A, and the expression in group C decreased significantly in comparison with that in group B (P<0.05). Conclusion As the recognition receptors of native immune system, TLR2 in the rejected corneal grafts may recognize the allograft antigen and play a role in acute graft rejection after penetrating keratoplasty.关键词
TLR2/角膜/排斥反应/实时荧光定量PCRKey words
Toll-like receptor 2/ cornea/ allograft rejection/ fluorescence quantitative real-time polymerase chain reaction分类
医药卫生引用本文复制引用
白浪,陆晓和,钟彦彦,周瑾,张静,武海军..TLR2在大鼠穿透性角膜移植术后免疫排斥反应中的作用[J].南方医科大学学报,2009,29(10):2051-2054,4.
