南方医科大学学报2009，Vol.29Issue(10)：1990-1993,4.
siRNA沉默COX-2基因对人卵巢癌细胞的增殖及迁移能力的影响
Small interfering RNA-mediated COX-2 gene silencing inhibits the proliferation and migration of human ovarian cancer cell line CAOV-3
摘要
Abstract
Objective To investigate the changes in the proliferation and migration of human ovarian cancer cells (CAOV-3) after knocking down COX-2 gene by RNA interference. Methods The recombinant plasmid of pGenesil-l-siRNA-COX-2 was constructed and transfected into CAOV-3 cells. The transcription of COX-2 gene was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression of COX-2 was determined by Western blotting . MTT assay was used to investigate the proliferation of the transfected CAOV-3 cells, and the cell migration was evaluated using a transwell migration assay. Results COX-2 mRNA and protein levels were significantly reduced after pGenesil-l-siRNA-COX-2 transfection into CAOV-3 cells, which showed obvious reduction in the cell proliferation and migration. Conclusion RNA interference allows obvious COX-2 gene knocking down in human ovarian cancer cells to result in lowered cell growth rate and migration ability. COX-2 gene may become a new therapeutic target for ovarian cancer.关键词
卵巢癌/环氧化酶-2/RNA干扰/肿瘤生成Key words
ovarian cancer/ cyclooxygenase 2/ RNA interference/ tumorgenesis分类
医药卫生引用本文复制引用
苏亚娟,王前,高玲娟,郑磊,包杰..siRNA沉默COX-2基因对人卵巢癌细胞的增殖及迁移能力的影响[J].南方医科大学学报,2009,29(10):1990-1993,4.基金项目
卫生部科研基金"荧光原位杂交技术在产前遗传性疾病及部分肿瘤检测的多中心临床应用研究"(WKJ2007-3-001) （WKJ2007-3-001）
