南方医科大学学报2009，Vol.29Issue(11)：2165-2167,2170,4.
彗星分析法研究靶向SMPD1的siRNA对小鼠卵母细胞凋亡的保护作用
siRNAs targetting sphingomyelin phosphodiesterase 1 protect mouse oocytes from apoptosis
摘要
Abstract
Objective To investigate the potential of siRNAs targeting sphingomyelin phosphodiesterase 1 (SMPD1) in protecting the oocytes from apoptosis, and explore new approaches to female fertility preservation. Methods Chemically synthesized siRNA targeting SMPD1 were introduced into mouse oocytes retrieved by hyperstimulation, and the cell apoptosis was analyzed by comic assay 48 and 72 h later. Results In the occytes without any siRNA injection, occyte DNA damage occurred after 24 h, and large amount of DNA fragments migrated from the cells 48 h later. In occytes injected with siRNA003, DNA migration decreased significantly as compared with the control and the other two groups injected with siRNA001 and siRNA002 (P<0.01). Conclusion siRNA targeting SMPD1 may protect the oocytes from apoptosis, and has the potential for use in future female fertility preservation.关键词
酸性鞘磷脂酶1/卵母细胞/细胞凋亡/RNA干扰/彗星分析法Key words
sphingomyelin phosphodiesterase/ oocytes/ apoptosis/ RNA/ Comet assay分类
医药卫生引用本文复制引用
张仁礼,陈系古,孟锦绣,闻安民,黄跃深,周灿权,王凯,贾磊,刘彩霞,邓新燕..彗星分析法研究靶向SMPD1的siRNA对小鼠卵母细胞凋亡的保护作用[J].南方医科大学学报,2009,29(11):2165-2167,2170,4.基金项目
国家自然科学基金(30000474 （）
30700327) （）
广东省自然科学基金(4009374 （）
9151009101000011 （）
7300047) （）
广东省医学科研基金(B2004038 （）
B2006003) （）
广东省人口计生委科研项目(2009227) （2009227）
