南方医科大学学报2009，Vol.29Issue(11)：2201-2204,4.
rAAV介导hBDNF基因转染对急性高眼压兔眼视网膜BDNF表达的影响
Effects of rAAV-mediated rhBDNF gene transfection on BDNF gene expression in the retina of a rabbit model of acute high intraocular pressure
摘要
Abstract
Objective To observe the changes in the expression of brain derived neurotrophic factor (BDNF) gene in the retina of rabbits with acute high intraocular pressure (IOP) after injection of recombinant adeno-associated virus (rAAV) vector containing human BDNF gene (rAAV-hBDNF), and investigate the neuroprotective mechanism of rAAV-hBDNF. Methods The unilateral eyes of 24 white rabbits were randomly chosen as the model group with high IOP induced by saline perfusion into the anterior chamber, and the contralateral eyes served as the control group without treatment. In another 24 white rabbits, 10 μl rAAV-BDNF was injected into the vitreous body of one of the eyes 3 days before induction of high IOP. On days 1,3,7, and 14 after perfusion, the bilateral eyes of 6 rabbits were excised for immunohistochemistry for the expression of endogenous BDNF gene in the retina. Results The number of BDNF-positive cells in the retina decreased after induction of high IOP, and injection of rAAV-hBDNF resulted in a significant increase in BDNF-positive cells as compared with the positive cell number in the high IOP model and control groups (P<0.05, P<0.01). Conclusion rAAV-mediated BDNF gene transfection can increase endogenous BDNF expression in the retina of rabbits with acute high IOP. Intravitreous injection is an effective pathway for rAAV-hBDNF gene transfection into the retina.关键词
脑源性神经营养因子/腺伴随病毒/高眼压/视网膜/基因表达Key words
brain derived neurotrophic factor/ adeno-associated virus/ high intraocular pressure/ retina/ gene expression分类
医药卫生引用本文复制引用
王建明,孙乃学,惠娜,范雅稚,冯海晓,赵世平..rAAV介导hBDNF基因转染对急性高眼压兔眼视网膜BDNF表达的影响[J].南方医科大学学报,2009,29(11):2201-2204,4.基金项目
陕西省自然科学基金(200lSM66) （200lSM66）
西安交通大学医学院第二附属医院科研基金 （）
