南方医科大学学报2009，Vol.29Issue(11)：2231-2232,2236,3.
老年大鼠供肝应用的实验研究
Application of aging donors in rat liver transplantation
摘要
Abstract
Objective To investigate the application of donor livers from aging rats, and discuss the age limit of the donor rats liver transplantation. Methods Arterialized, two-cuff technique orthotopic liver transplantation was performed in male Wistar rats. All the recipient rats were 5 months old, and the ages of the donor rats were 5 (group A), 17 (group B), 20 (group C), 23 (group D), and 26 (group E) months (n=12). The postoperative function recovery and pathological changes of the liver grafts were evaluated by serum alanine aminotransferase (ALT) detection and histopathological examination, and the 3-month survival rate of the rats was observed. Results Aging liver grafts in groups B, C, and D caused early elevation of ALT peak level and aggravation of liver tissue damage, and the liver graft recovery was delayed until postoperative day 7. Mild liver fibrosis, reduced hepatocytes and pigment deposition were observed in the liver grafts before the transplantation. Compared with the other groups, the rats in group E showed significantly increased ALT levels after the transplantation (P<0.05), with failure of liver graft function recovery and significantly reduced 3-month survival rate (0%, P<0.05). Conclusion The donor age of the rats is a crucial factor to affect the outcome of the liver grafts. Grafts obtained from rats younger than 23 months allow better functional recovery of the liver.关键词
肝移植/老年大鼠/供体年龄/丙氨酸转氨酶Key words
liver transplantation/ aging rats/ donor age/ alanine aminotransferase分类
医药卫生引用本文复制引用
许赤,易慧敏,傅斌生,李华,张彤,陈规划..老年大鼠供肝应用的实验研究[J].南方医科大学学报,2009,29(11):2231-2232,2236,3.基金项目
广东省自然科学基金(6300763) （6300763）
