南方医科大学学报2009，Vol.29Issue(11)：2241-2243,3.
不同放射敏感性肺腺癌细胞株中DNA-PKcs蛋白的表达情况比较
Comparison of DNA-dependent protein kinase catalytic subunit expression in two lung adenocarcinoma cell lines with different radiosensitivity
摘要
Abstract
Objective To investigate DNA-dependent protein kinase catalytic subunit (DNA-PKcs) content and activity in lung adenocarcinoma cell lines and its correlation with radiosensitivity. Methods The content and activity of DNA-PKcs were analyzed in two lung adenocarcinoma cell lines A549 and H1299 by Western blotting and the Signa TECT DNA-PK assay kit. The dose-survival relationship for two cell lines was analyzed using clonogenic formation assay. Results A549 was more radiosensitive than H1299. The survival fractions at 2 Gy (SF2) were 0.7412 in A549 cell line and 0.2473 in H1299 cell line. The content of DNA-PKcs was significantly higher in A549 cells than in H1299 cells (t=10.37, P<0.001). The integrated optical densities were 3.29±0.44 in A549 cells and 0.50±0.17 in H1299 cells. DNA-PKcs activities in A549 and H1299 cells were 8.29±1.37 and 2.47±1.09, respectively, showing a significant difference between them (t=5.76, P=0.005). Conclusion DNA-PKcs is an important factor to affect the radiosensitivity of lung adenocarcinoma cell lines.关键词
DNA-PKcs/肺腺癌/放射敏感性Key words
DNA-dependent protein kinase catalytic subunit/ adenocarcinoma/ radiosensitivity分类
医药卫生引用本文复制引用
岑伟健,潘焱,李伟雄,杨素清..不同放射敏感性肺腺癌细胞株中DNA-PKcs蛋白的表达情况比较[J].南方医科大学学报,2009,29(11):2241-2243,3.基金项目
广东省医学科学技术研究基金(A2006014) （A2006014）
广东省自然科学基金(06020905) （06020905）
