南方医科大学学报2009，Vol.29Issue(10)：1969-1972,4.
水溶性乌梢蛇总蛋白对成纤维样滑膜细胞分泌白介素-1β、α-肿瘤坏死因子和白介素-10的影响
Effect of soluble total proteins of Zaocys dumnades on interleukin-1β,tumor necrosis factor-αand interleukin-10 expressions in human fibroblast-like synoviocytes in vitro
摘要
Abstract
Objective To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. Methods Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 μg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect the expressions of IL-1β, IL-10 and TNF-α in the FLS. Results The protein and mRNA levels of IL-1β and TNF-α in the supernatant of the FLS exposed to 150 and 450 μg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). Conclusion The soluble total proteins of Zaocys dhumnades produce therapeutic effect on RA possibly by inhibiting IL-1β and TNF-α and promoting IL-10 expressions in the FLS.关键词
水溶性乌梢蛇总蛋白/成纤维样滑膜细胞/白介素-1/肿瘤坏死因子/白介素-1OKey words
soluble total protein of Zaocys dumnades/ fibroblast-like synoviocytes/ interleukin-1β/ tumor necrosis factor-α/ interleukin-10分类
医药卫生引用本文复制引用
吴贺勇,李娟,李亚玲..水溶性乌梢蛇总蛋白对成纤维样滑膜细胞分泌白介素-1β、α-肿瘤坏死因子和白介素-10的影响[J].南方医科大学学报,2009,29(10):1969-1972,4.基金项目
国家自然科学基金面上项目(No:30572457) （No:30572457）
