福建农林大学学报（自然科学版）2009，Vol.38Issue(5)：512-516,5.
一株新城疫病毒HN基因的克隆及序列分析
Cloning and sequence analysis of HN gene of a Newcastle disease virus isolate
摘要
Abstract
The HN gene of Newcastle disease virus isolate (NDV) JZ05 was amplified with reverse transcription-polymerase chain reaction (RT-PCR) , its gene cloning was completed, and its nucleotide sequence was determined. Alignment was executed to the HN gene and its amino acids sequence between NDV isolate JZ05 and other 18 NDV isolates by a sequence analysis software ( namely DNAssist). The result of sequence determining to the HN gene showed that there were 1716 bp in the HN gene, and in HN protein there were 571 amino acid residues, 5 sites of potential carbohydrate binding, 13 cysteine residues, no sixth site (538-540 aa). In HN protein of NDV isolate JZ05 single amino acid residue of 6 sites became different as compared with other 18 NDV isolates. Homology was 94% over for the HN gene nucleotide sequence and amino acid sequence between strain JZ05 and 11 strains (ZJ1, TJ03, Jlan-1-00, SBZ02, SCL03, JS02, GD05, SQD04, SQZ04, YG, Taiwan 95) , but homology was about 82% for HN gene nucleotide sequence and about 84% for its amino acid sequence between strains JZ05 and 3 vaccine strains (Clone30, LaSota, B1).关键词
新城疫病毒/HN基因/基因克隆/序列分析Key words
Newcastle disease virus/ HN gene/ cloning/ sequence analysis分类
农业科技引用本文复制引用
程太平,荣俊,刘超..一株新城疫病毒HN基因的克隆及序列分析[J].福建农林大学学报（自然科学版）,2009,38(5):512-516,5.基金项目
湖北省教育厅资助项目(D200612005). （D200612005）
