福建农林大学学报（自然科学版）2009，Vol.38Issue(5)：517-520,4.
姬松茸多酚氧化酶的动力学特性
Kinetics of polyphenol oxidase in Agaricus blazei
刘文聪1
作者信息
- 1. 福建农林大学食品科学技术研究所,福建,福州,350002
- 折叠
摘要
Abstract
The polyphenol oxidase (PPO) activities in different parts of Agaricus blazei Murrill were determined, the effects of pH value, temperature, substrate concentration and enzyme inhibitors on PPO were studied, too. The results showed that the optimum pH value and optimum temperature for PPO were pH 6.4 and 30 ℃, respectively, the Michaelis constant (K_m) and V_(max) of PPO with pyrocatechol substrate were 6.23 × 10~(-3) mol · L~(-1) and 1.42 mol · L~(-1) · min~(-1) respectively using catechol as substrates, and the reaction kinetic equation was established. The inhibition of citric acid on PPO was not satisfactory, NaHSO_3 and sodium d-isoascorbate could inhibit effectively, and the effective concentrations were 0.18% and 0.21% , respectively.关键词
姬松茸/多酚氧化酶/动力学特性Key words
Agaricus blazei/ polyphenol oxidase/ kinetic characteristics分类
轻工纺织引用本文复制引用
刘文聪..姬松茸多酚氧化酶的动力学特性[J].福建农林大学学报（自然科学版）,2009,38(5):517-520,4.
