福建农林大学学报（自然科学版）2009，Vol.38Issue(5)：521-525,5.
1型和2型鸭疫里默氏菌的交叉保护
The cross-protection between Riemerella anatipestifer serotype 1 and 2
摘要
Abstract
The sequences of 16S rDNA and 16S-23S rDNA intergenic spacers of 29 strains of Riemerella anatipestifer (RA) were determined. Phylogenetic analysis indicated that the serotype 1 strains and the majority of serotype 2 strains formed different branches , whereas serotype 2 strains RAfl 1, RAf3 and RAf104 were found to be closely related to serotype 1. Inactivated vaccines of RA serotype 2 were prepared using strain RAfl9 in serotype 2 branch and strain RAfl 1 in serotype 1 branch, respectively. After immunization, the ducks were challenged with serotype 1 and 2 strains. The vaccine prepared using strain RAfl1 produced a higher degree cross-protection (53.8% ) against challenge with serotype 1 strain. In contrast, the vaccine prepared using strain RAfl9 produced cross-protection of 23.1% against challenge with serotype 1 strain. Using phylogenetic analysis of the sequences of 16S rDNA and 16S -23S rDNA intergenic spacers, a strain of RA with cross-protection was found.关键词
鸭疫里默氏菌/16SrRNA序列/16S-23SrRNA间隔区序列/交叉保护Key words
Riemerella anatipestifer/ 16S rRNA genes/ 16S-23S rRNA intergenic spacer sequence/ cross-protection分类
农业科技引用本文复制引用
程龙飞,傅光华,彭春香,施少华,黄瑜,陈红梅,万春和..1型和2型鸭疫里默氏菌的交叉保护[J].福建农林大学学报（自然科学版）,2009,38(5):521-525,5.基金项目
福建省自然科学基金资助项目(B0310025) （B0310025）
福建省科技厅重大专项资助项目(2006Z0003-2) （2006Z0003-2）
福建省科技厅科技计划项目(2006N0025) （2006N0025）
福建省财政厅专项资助项目(STIF-Y02). （STIF-Y02）
