福建林学院学报2009，Vol.29Issue(4)：315-319,5.
雷公藤优良无性系组织培养技术的研究
Study on tissue culture conditions and technology of Tripterygium wilfordii superior clone
摘要
Abstract
By orthogonal experiment the tender green leaves were used as explants to study the effect of different medium on adventitious buds induction, adventitious buds proliferation and rooting experiment of Tripterygium wilfordii superior clone. The results showed that there was a significant impact to T.wilfordii adventitious buds induction cultivated on different concentrations of hormone combinations. The optimal induction medium was MS+IAA 0.2 mg·L~(-1)+KT 0.5 mg·L~(-1)+6-BA 1.5 mg·L~(-1). There was a very significant impact to T.wilfordii adventitious buds proliferation cultivated on different concentrations of hormone combinations. The optimal proliferation medium was MS+NAA 0.1 mg·L~(-1)+KT 0.1 mg·L~(-1)+6-BA 1.0 mg·L~(-1). There was not significant impact to T.wilfordii rooting cultivated in different treatments, the adaptive rooting medium was 1/2MS+NAA 2.0 mg·L~(-1)+KT 0.1 mg·L~(-1).关键词
雷公藤/不定芽诱导/不定芽增殖/生根培养Key words
Tripterygium wilfordii/adventitious buds induction/adventitious buds proliferation/rooting cultivation分类
林学引用本文复制引用
李建鹃,洪伟,吴承祯,谢安强,李键,林晗..雷公藤优良无性系组织培养技术的研究[J].福建林学院学报,2009,29(4):315-319,5.基金项目
国家教育部博士点基金资助项目(20061038901) （20061038901）
福建省科技重大专项资助项目(2006NZ0001). （2006NZ0001）
