福建师范大学学报（自然科学版）2009，Vol.25Issue(6)：65-70,6.
丁草胺对中华大蟾蜍蝌蚪的遗传毒性
Genotoxicity of the Herbicide Butachlor on Bufo gargarizans gargarizans Tadpoles
摘要
Abstract
Micro-nuclear test and the alkaline single-cell gel electrophoresis assay (SCGE) or comet assay was performed to examine the genotoxicity of butachlor on erythro-cytes of Bufo gargarizans gargarizans tadpoles exposed to increasing concentration of the tested substance (0.1,0.2,0.3,0.4 mg/L). The results showed that both micro-nuclear frequency and nuclear abnormity frequency were significantly increased with the increasing concentration of butachlor in laboratory condition. Tadpoles treated for 24 h had significant, dose-responsive increases in the levels of DNA damage, as measured by the mean DNA length: width ratio. There were strong linear correlations between cells damage frequency, the mean DNA damage (length-to-width ratios) and the concentrations of butachlor. The results of this study indicate that butachlor produce genotoxicity in amphibians, and that using micro-nuclear test and SCGE may be useful for measuring DNA damage in tadpoles exposed in the field.关键词
中华大蟾蜍蝌蚪/丁草胺/遗传毒性/微核实验/彗星实验Key words
Bufo gargarizans gargarizans tadpole/ butachlor/ genotoxicity/ micro-nuclear test/ comet assay分类
生物科学引用本文复制引用
林玲,姚丹,钟碧瑾,房振华,王楠楠,耿艳,耿宝荣..丁草胺对中华大蟾蜍蝌蚪的遗传毒性[J].福建师范大学学报（自然科学版）,2009,25(6):65-70,6.基金项目
福建省科技厅基金资助项目(2007F5033) （2007F5033）
福建省自然科学基金资助项目(2009J01129) （2009J01129）
