分析测试学报2009，Vol.28Issue(11)：1266-1269,1274,5.DOI:10.3969/j.issn.1004-4957.2009.11.008
IEX/RP二维液相色谱中弱保留组分收集模式的构建及系统评价
Construction and Evaluation of Weak Retention Components Collection Mode - IEX/RP Two-Dimensional Liquid Chromatography System
摘要
Abstract
An ion-exchange/reversed phase liquid chromatography(2D-LC) system was developed based on trap column and valve interface. Weak anion exchange ( WAX ) chromatography mode was used in the first dimension and reversed-phase(RP) chromatography mode was used in the second di-mension. The two dimensions were connected by a 10-port two-position valve and two trap columns. The weak retained components of the first-dimensional were collected and analyzed separately, and then the remained components were further separated by 2D-LC system. The overloading of the sec-ond-dimensional column was effectively avoided and the separation power of the 2D-LC system was improved greatly. Tryptic digest of four proteins were separated on this system. 115 peaks were identified in the traditional 2D-LC separation mode. However, in the weak retention components collection mode, 58 peaks were detected in the front 15 min elution of the first dimension. 78 peaks were detected in the subsequent 2D-LC analysis. Compared with that of traditional 2D-LC, another 21 peaks were identified and some low abundance components were identified after weak retained components were collected.关键词
二维液相色谱系统/离子交换色谱/反相色谱/蛋白质/弱保留组分收集模式Key words
two-dimensional liquid chromatographic system/ ion exchange chromatography/ reversed-phase liquid chromatography/ peptide/ weak retention components collection mode分类
化学化工引用本文复制引用
宋伦,李笃信,张凌怡,杜一平,张维冰..IEX/RP二维液相色谱中弱保留组分收集模式的构建及系统评价[J].分析测试学报,2009,28(11):1266-1269,1274,5.基金项目
国家自然科学基金资助项目(20675083) （20675083）
国家重点基础研究发展规划(973)资助项目(o1CB5ro202) （973）
科技部 "十一五"国家科技支撑计划重大项目资助(2006BAF07B03) （2006BAF07B03）
