分析化学2009，Vol.37Issue(11)：1667-1670,4.
反相高效液相色谱法测定牛奶中的主要蛋白质
Determination of Major Bovine Milk Proteins by Reversed Phase High Performance Liquid Chromatography
摘要
Abstract
A RP-HPLC method for the quantification of the six major bovine milk proteins (κ-casein (CN),α_(s2)-CN,α_(s1)-CN,β-CN,Whey,immunoglobulins (Igg) ) is described. Separation and quantification were achieved by a reversed phase analytical column (Agilent Zorbax 300SB-C_8,250 mm×4.6 mm ,5μm) and the gradient elution solvents of 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in acetonitrile at a flow rate of 0. 8 mL/min. Column temperature was set at 45℃ and the sample was monitored with photodiode array detector at 214 nm. A linear relationship( r >0. 999) between the concentrations of proteins and peak areas was observed over the concentration range. Recoveries of six target proteins spiked in milk were form 74. 8% to 132.5%. Nine kinds of milks of different brands were analyzed,and the difference of the concentration and relative ration of κ-CN,α_(s2)-CN,α_(s1)-CN,β-CN and Whey were found.关键词
高效液相色谱/牛奶/蛋白质Key words
Reversed phase high performance liquid chromatography/bovine milk/protein分类
化学化工引用本文复制引用
王娟,张庆合,王志华,李红梅..反相高效液相色谱法测定牛奶中的主要蛋白质[J].分析化学,2009,37(11):1667-1670,4.基金项目
本文系国家自然科学基金(No.20775075)和国家科技支撑计划(No.2006BAF07B03)资助项目 （No.20775075）
