高等学校化学学报2009，Vol.30Issue(11)：2175-2179,5.
吡蚜酮与牛血清白蛋白的相互作用
Interaction Between Pymetrozine and Bovine Serum Albumin
摘要
Abstract
The interaction between pymetrozine (Py) and bovine serum albumin ( BSA) was investigated by spectroscopy methods, including fluorescence, ultraviolet absorption (UV) and far-UV circular dichroism (CD) spectroscopies. The quenching mechanism of fluorescence was suggested as static quenching according to the Stern-Volmer equation. The thermodynamic parameters enthalpy change(△H) and entropy change(△S) were calculated, which suggested that the binding power between pymetrozine and bovine serum albumin was hydrogen-bond and van der Waals force. According to the Forster non-radiation energy transfer theory, the binding average distance (r =2.4 nm) between donor (BSA) and acceptor(Py) was obtained. Furthermore, the investigations of the synchromous fluorescence and CD spectra of the system reveal that the conformation of BSA is changed in the presence of Py.关键词
吡蚜酮/牛血清白蛋白/荧光猝灭/相互作用Key words
Pymetrozine/ Bovine serum albumin/ Fluorescence quenching/ Interaction分类
化学化工引用本文复制引用
徐巍,吴霞,周海平,刘潇彧,杨景和,范金勇,张梅凤..吡蚜酮与牛血清白蛋白的相互作用[J].高等学校化学学报,2009,30(11):2175-2179,5.基金项目
国家自然科学基金(批准号: 20575035)和山东省自然科学基金(批准号: Z2008B04)资助. （批准号: 20575035）
