广东医学2009，Vol.30Issue(11)：1600-1603,4.
E2F-1过表达对胃癌细胞生物学行为的影响
The molecular mechanism of the effects of E2F -1 overexpression on biological characteristics of human gastric carcinoma cell line
摘要
Abstract
Objective To study the molecular mechanism of the effects of E2F -1 overexpression on biological characteristics of human gastric carcinoma cell line with eDNA microarray technique. Methods Total RNA were extrac-ted from gastric cancer cell line MGC -803 transfeeted with E2F -1 and then purified. The eDNA obtained by reverse transcription polymerase chain reaction (RT-PCR) ,was labeled with Cy5 and Cy3 fluorescence as probes, and then hy-bridized with gene chip containing 21522 human 22K gene expression profile. Subsequently, the two signal images were scanned by LuxSean 10K/A dual pathways laser scanner and analyzed by LuxScan3.0 image analysis software. We ran-domly chose three of the differentially expressed genes to confirm the array results using semi -quantitative RT -PCR. Results Of the 21522 target genes, 740 genes were screened out for differences in gene expression level. 405 of the 740 genes were up -regulated and 335 were down -regulated. 73 of differentially expressed genes were with unknown func-tion. The results of RT -PCR were well coincided with the cDNA microarray results. Conclusion The carcinogenesis of gastric carcinoma includes the interaction of multiple genes and regulation of many signal transduction pathways. E2F -1 can affect the biological characteristics of MGC -803 by TP53 signal pathway, and is closely associated with the differenti-ally expressed genes.关键词
胃癌/E2F-1/基因表达谱芯片/差异表达基因Key words
gastric adenocarcinoma/ E2F -1 / gene expression profile/ differentially expressed genes分类
医药卫生引用本文复制引用
王晓通,李雷,肖强,谢玉波,王长青..E2F-1过表达对胃癌细胞生物学行为的影响[J].广东医学,2009,30(11):1600-1603,4.基金项目
国家自然科学基金资助项目(编号:30860273) （编号:30860273）
