果树学报2009，Vol.26Issue(5)：632-637,6.
草莓不同器官中microRNA表达差异研究
Study on the difference in expression profiles of microRNA among different organs of strawberry
摘要
Abstract
MicroRNA (miRNA) plays an important role in plant growth and development. Using total RNA extracted by CTAB method as templates, the expression profiles of 6 miRNA in root, receptacle, shoot tip and leaf of strawberry were studied by real-time RT-PCR through stem-loop RT primers. When the relative quantity of 6 miRNA in strawberry leaf was as 1, the result of different expression showed that the highest expression in receptacle was miR164, reaching 6.615, obviously higher than the quantity in root (0.485), shoot tip (0.371) and leaf (1). While the expression of miR172 in shoot tip and receptacle were 0.029 and 0.020, lower than that in leaf. The lowest expression of miR172 was in root, only 0.000 845 4, the same as miR167, only 0.053. No obvious difference in expressions of miRi56, miR159 and miR165 was found in 4 kinds of strawberry organs.关键词
草莓/MicroRNA/Real time RT-PCR/表达/器官Key words
Strawberry/ MicroRNA/ Real time RT-PCR/ Expression/ Organ分类
农业科技引用本文复制引用
李贺,印东升,王志刚,黄飞飞,常琳琳,张志宏..草莓不同器官中microRNA表达差异研究[J].果树学报,2009,26(5):632-637,6.基金项目
国家自然科学基金(30671432) （30671432）
教育部新世纪优秀人才支持计划 （）
沈阳农业大学青年教师科研基金 （）
