果树学报2009，Vol.26Issue(5)：659-665,7.
菠萝蜜遗传多样性的ISSR分析
Analysis of genetic diversity of Jackfruit germplasm using ISSR marking method
摘要
Abstract
ISSR (inter-simple sequence repeat)markers were used to analyze genetic diversity in 76 accessions of jackfruit (Artocarpus heterophyllus Lain.). Among 477 bands produced by 24 ISSR primers, 427 were polymorphic (accounted for 89.52%). The average PIC (pelymorphism information content) was 0.23. These 76 jackfruit accessions could be discriminated by the 24 ISSR primers. Genetic distance analysis revealed that, low genetic diversity existed in the jackfruit germplasm studied, and the genetic similarity coefficient ranged from 0.626 to 0.945, with an average of 0.775. Cluster analysis showed that 4 groups could be clustered at a genetic distance coefficient of 0.752. The results indicated that, 2 accessions from Tropical Plant Garden, WJ2 and GSYWJ1, were genetically different from other accessions. Accessions of soft flesh type and firm flesh type could not be discriminated. Accessions collected from different sources could form independent clusters, while accessions collected from different areas of Leizhou peninsula could not form independent clusters.关键词
菠萝蜜/种质资源/遗传多样性/ISSRKey words
Jackfruit/ Germplasm resources/ Genetic diversity/ ISSR分类
农业科技引用本文复制引用
叶春海,王耀辉,李映志,丰锋..菠萝蜜遗传多样性的ISSR分析[J].果树学报,2009,26(5):659-665,7.基金项目
国家自然科学基金(30771484) （30771484）
广东省自然科学基金(7010103) （7010103）
广东省农业领域科技计划重大项目(2007A020200006) （2007A020200006）
