果树学报2009，Vol.26Issue(5)：748-751,4.
运用实时荧光RT-PCR技术检测柑橘碎叶病毒
Detection of Citrus tatter leaf virus (CTLV) by real-time RT-PCR
摘要
Abstract
Citrus tatter leaf disease caused by citrus tatter leaf virus (CTLV) is an economically important systemic disease of citrus in the world. So it is essential to develop a rapid and reliable method to detect CTLV. Using a pair of primers ASG-Pf and ASG-Pr, a real-time RT-PCR system based on SYBR Green Ⅰ dye has been established to detect CTLV. The specificity, sensitivity and applicability of this system were detected. The results showed that only CTLV could be detected, while Citrus tristeza virus (CTV), Satsuma dwarf virus (SDV)and Citrus psorosis virus (CPV) can not be detected. The sensitivity of real-time PCR is higher than that of conventional PCR by 100 fold, and it can detect CTLV isolates from different kinds of citrus cuhivars. Real-time RT-PCR doesn't need post-PCR handling of the amplified products such as electrophoresis, staining and imaging by completing the whole process within a single tube, and SYBR Green Ⅰ dye needs low cost, so it is suitable to detect CTLV.关键词
柑橘碎叶病毒(CTLV)/实时荧光RT-PCR/检测Key words
Citrus tatter leaf virus (CTLV) / Real-time RT-PCR / Detection分类
农业科技引用本文复制引用
刘科宏,周常勇,宋震,周彦,李中安,唐科志..运用实时荧光RT-PCR技术检测柑橘碎叶病毒[J].果树学报,2009,26(5):748-751,4.基金项目
国家支撑计划(2007BAD47B03,2007BAD61B04) （2007BAD47B03,2007BAD61B04）
重庆市自然科学基金(2007BBB44) （2007BBB44）
