果树学报2009，Vol.26Issue(5)：752-755,4.
桃叶片总蛋白双向电泳技术的研究
Study of two-dimensional gel electrophoresis for total proteins from peach leaves
杜保伟 1李成斌 2熊明国 1李靖1
作者信息
- 1. 河南农业大学,郑州,450002
- 2. 商丘职业技术学院,河南,商丘,476000
- 折叠
摘要
Abstract
Two-dimensional gel electrophoresis(2-DE) was used to seek out the procedure suitable for leaf proteome analysis of peach. Two sample preparation methods, two different pH gradients and three different sample amounts were compared. The result showed that good 2-DE gels obtained by the procedure of TCA/acetone precipitation, ampholine pH3～10:pH 5～7 in IEF gels according the ratio of 1:4, 90 μg loaded sample combined with suitable electrophoresis parameter. This improved 2-DE system resulted in a protein profile of better resolution and higher protein yields as analyzed by Image Master 2D soft-ware. Good results were also obtained by MS/MS.关键词
桃/叶片/蛋白质/双向电泳Key words
Peach/ Leaves/ Protein/ Two-dimensional gel electrophoresis/ 2-DE分类
农业科技引用本文复制引用
杜保伟,李成斌,熊明国,李靖..桃叶片总蛋白双向电泳技术的研究[J].果树学报,2009,26(5):752-755,4.
