医学分子生物学杂志2009，Vol.6Issue(6)：494-497,4.DOI:10.3870/j.issn.1672-8009.2009.06.005
荧光定量PCR测定端粒长度
Telomere Measurement by Quantitative PCR
摘要
Abstract
Objective To determine relative telomere length by quantitative PCR.Methods Genomic DNA was extracted from 9 types of human cells.Relative T/S ratios measured by quantitative PCR were compared with relative mean terminal restriction fragment(TRF)lengths in the same samples measured by traditional Southern blot methods.Results Relative R/S ratio measured by Q-PCR was 0.68±0.57,and relative mean TRF length was 8.57±2.34.By linear regression analysis,the correlation coefficient,R~2,for the relationship of T/S ratio to TRF length,was 0.7807(P＜0.001).Conclusion We have demonstrated the measurement of relative average telomere lengths by quantitative PCR,using a carefully designed pair of oligonucleotide primers.The assay is simple,rapid and reproducible,thus reliable for a high throughput of samples.It is recommendable to be used in study of telomere biology and genetic epidemiology of cancer and aging related diseases.关键词
荧光定量聚合酶链式反应/端粒长度测定/T/S比率Key words
fluorescence quantitative PCR/determination of telomere length/T/S ratios分类
医药卫生引用本文复制引用
王敏敏,陈莹,王慧萍,冯时,王宇成,茅幼英,姜虹,陈江华,杨浩,刘广义,陈大进,陈文清,蔡洁茹,周芹,谢易,陈芷珉..荧光定量PCR测定端粒长度[J].医学分子生物学杂志,2009,6(6):494-497,4.基金项目
国家自然科学基金(No.30671990,30900689) （No.30671990,30900689）
