华北农学报2009，Vol.24Issue(5)：1-6,6.
小麦芽生长点的基因枪转化技术研究
Research on Shoot Apical Point Transformation of Wheat Germinated Seeds by Particle Bombardment
摘要
Abstract
In order to set up a simple and easy method for genetic transformation of wheat, which do not depend on tissue culture and resistance selection, experiments of target-preparation of shoot apical point of buds for particle delivery and parameter-optimization of bombardment were conducted with a cultivar of Shi4185 as a recipient, and with gus, badh/bar as foreign genes.A suitable way was successfully founded to prepare shoot apical point targets from germinated seeds of wheat to bombardment, and the optimum parameters combination was determined as 1 100 Psi + 9 cm. Statistics of gus detection of transient expression with 70×3 batches indicated that 22.9 % - 35.7 % of targets and 5.7% -8.6% of shoot apical points were transformed. With the bombardment of pABH9 containing badh/bar, 802/1 000 targets devel-oped to normal plants. 105 resistant T_1 plants were obtained by spraying PPT of 200 ppm to the seedlings of seeds corre-sponding to 42 T_0 plants with a resistance rate of 5.2 % . PCR analysis showed that 6 T_1 plants were positive. Preliminari-ly, the technique to transform shoot apical point of wheat buds by particle bombardment was established.关键词
小麦/芽生长点/基因枪/转化Key words
Wheat/ Shoot apical point/ Particle bombardment/ Transformation分类
农业科技引用本文复制引用
董福双,张艳敏,杨帆,梁新朝,刘桂如,王海波..小麦芽生长点的基因枪转化技术研究[J].华北农学报,2009,24(5):1-6,6.基金项目
转基因生物新品种培育重大专项(2009ZX08010-011B) （2009ZX08010-011B）
