华北农学报2009，Vol.24Issue(5)：31-34,4.
种子特异启动子的GPD1基因植物表达载体构建
Construction of A Seed-specific Plant Expression Vector Targeting the GPD1 Gene
摘要
Abstract
By the PCR technique, seed-specific promoter (napin) and glycerol-3-phosphate dehydrogenase( GPD1) were amplified from Brassica napus and Saccharomyces cerevisiae INVScl genomic DNA,and cloned into the pMD18-T vector, respectively .The two fragments were inserted into the multiple cloning sites of the binary vector pCAMBIA1390 us-ing the pBluescriptKS as the intermediary cloning vector. The resulted seed-specific expression vector was named as pl390NG.The vector was transformed into the Agrobacterium tumefaciens strains LBA4404,EHA105 and AGL1. It may provide an excellent genetic resources for genetic improvement of the seed oil content of energy plant.关键词
甘蓝型油菜/酵母INVSc1/Napin/3-磷酸甘油脱氢酶(GPD1)/基因表达载体Key words
Brassica napus/ Saccharomyces cerevisiae INVScl/ Napin/ Glycerol-3-phosphate dehydrogenase (GPDl)/Gene expression vector分类
生物科学引用本文复制引用
李群,王学英,刘野,阮成江..种子特异启动子的GPD1基因植物表达载体构建[J].华北农学报,2009,24(5):31-34,4.基金项目
国家"十一五"科技支撑计划重大项目子专题(2006BAD09A04) （2006BAD09A04）
中国博士后科学基金特别资助(200801371) （200801371）
中国博士后科学基金(20070420990) （20070420990）
江苏省博士后基金(0702018C) （0702018C）
